ISO 9308-1 FREE PDF

Incubation of the membrane filter at C for h. Counting of -D-galactosidase positive colonies pink to red as presumptive coliform bacteria that are not E. To avoid false-positive results, caused by oxidase positive bacteria, for example, Aeromonas spp, the presumptive colonies shall be confirmed by a negative oxidase reaction. Counting of -D-galactosidase and -D-glucuronidase positive colonies dark-blue to violet as E. Total coliform bacteria are the sum of oxidase negative colonies with pink to red colour and all dark-blue to violet colonies. The following are the usual microbiological laboratory equipment.

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Incubation of the membrane filter at C for h. Counting of -D-galactosidase positive colonies pink to red as presumptive coliform bacteria that are not E. To avoid false-positive results, caused by oxidase positive bacteria, for example, Aeromonas spp, the presumptive colonies shall be confirmed by a negative oxidase reaction. Counting of -D-galactosidase and -D-glucuronidase positive colonies dark-blue to violet as E. Total coliform bacteria are the sum of oxidase negative colonies with pink to red colour and all dark-blue to violet colonies.

The following are the usual microbiological laboratory equipment. The membrane filters shall be free from growth-inhibiting or growth-promoting properties and the printing ink used for the grid shall not affect the growth of bacteria. If not obtained sterile, they shall be sterilized according to the manufacturers instructions. Every batch of membrane filters shall be tested for its suitability for the test according to ISO especially since the use of different brands of membrane filters may result in different recovery and colour development.

Use ingredients of uniform quality and chemicals of analytical grade see note ; follow the instructions given in AnnexB. NOTE The use of chemicals of other grades is possible providing they are shown to be of equal performance in the test. For preparation of culture media, use distilled water or deionized water free from substances which might inhibit bacterial growth under the conditions of the test and which is in accordance with ISO Samples have to be transported and stored at 53 C in accordance with ISO Under exceptional circumstances, the samples may be kept at 53 C for up to 24h prior to examination.

In this case, the storage time has to be mentioned in the test report. The minimum volume for filtration is 10 ml of sample or dilutions thereof to ensure even distribution of the bacteria on the membrane filter. Examine the membrane filters and count all colonies giving a positive -D-galactosidase reaction pink to red as presumptive coliform bacteria that are not E.

Count all colonies giving a positive -D-galactosidase and -D-glucuronidase reaction dark-blue to violet as E. To confirm the presumptive coliform bacteria that are not E. Test preferentially all, or at least 10 pink to red colonies selected as described in ISO For this confirmation step, appropriate commercialized oxidase tests1 can be used. If commercial oxidase test is not used, the oxidase-test can be performed by adding two to three drops of fresh oxidase reagent B.

The colonies which have to be confirmed are transferred onto the pretreated filter paper using a plastic or platinum inoculating loop.

A positive oxidase reaction is shown by the appearance of a dark-blue colour within 30s. This shall not be observed for coliform bacteria since they are oxidase negative. If many colonies have grown on the membrane filter or if a presumptive colony is located next to other colonies, it might be necessary to prepare subcultures of the presumptive colonies to ensure that the oxidase test is carried out with pure cultures.

Bactident is an example of suitable product available commercially. This information is given for the convenience of the users of this International Standard and does not constitute an endorsement by ISO of this product. Subculture onto a non-selective agar e. The count of coliform bacteria is the sum of all oxidase negative pink to red colonies plus all dark-blue to violet colonies.

The use of positive controls, negative controls, and blanks is part of the test. Table1 shows the performance tests for CCA.

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ISO 9308-1:2014/Amd 1:2016

Differentiating chromogenic agar for the detection and bacterial count of E. Ltd Room No. Withoutabox Submit to Film Festivals. Amazon Advertising Find, attract, and engage customers. Sign In Not yet registered? The Tryptophan contained in the medium improves the indol reaction for the further confirmation of E. English Choose a language for shopping.

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